Reducing sugars can induce the oxidative inactivation of rhodanese.
نویسندگان
چکیده
منابع مشابه
Conformational changes accompany the oxidative inactivation of rhodanese by a variety of reagents.
Rhodanese is oxidatively inactivated by several reagents, some of which are not normally considered oxidants. Rhodanese, in a form not containing persulfide sulfur (E), was inactivated by phenylglyoxal under conditions where disulfides are formed. There was the concomitant increase in the fluorescence of the apolar probe 1,1'-bi(4-anilino)naphthalene-5,5'-disulfonic acid (bisANS). At 0.2 mg/ml ...
متن کاملOxidative inactivation of the enzyme rhodanese by reduced nicotinamide adenine dinucleotide.
The enzyme rhodanese (thiosulfate sulfurtransferase; EC 2.8.1.1) is inactivated with a half-time of approximately 3 min when incubated with 50 mM NADH. NAD+, however, has virtually no effect on the activity. Inactivation can be prevented by the inclusion of the substrate thiosulfate. The concentration of thiosulfate giving half-protection is 0.038 mM. In addition, NADH, but not NAD+, is a compe...
متن کاملThe Determination of Reducing Sugars
In the course of an investigation, still in progress, of the physiological activity of a group of soil organisms, it became necessary to determine the quantity of dextrose in a series of solutions. It was desirable to have a method as accurate as the Fehling gravimetric one but which would yield results about as quickly as by direct titration. The reducing sugar present in the 150 cc. of each o...
متن کاملOxidative inactivation of rhodanese by hydrogen peroxide produces states that show differential reactivation.
Controlled conditions have been found that give complete reactivation and long term stabilization of rhodanese (EC 2.8.1.1) after oxidative inactivation by hydrogen peroxide. Inactivated rhodanese was completely reactivated by reductants such as thioglycolic acid (TGA) (100 mM) and dithiothreitol (DTT) (100 mM) or the substrate thiosulfate (100 mM) if these reagents were added soon after inacti...
متن کاملCan mustard gas induce late onset polyneuropathy?
Abstract Background: Mustard gas, lethal in high doses, affects multiple organs such as skin, eye and respiratory system. We studied the development of late onset mustardinduced polyneuropathy among chemically wounded Iranian veterans. Methods: In this descriptive study, 100 chemically wounded Iranian veterans with severe eye involvement were examined for any signs and symptoms of polyneuropat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)35880-0